Genetic Diversity among Ten (10) Local Accessions of Tomato [Solanum Lycopersicum (Linn)] from the Southern Senatorial District of Adamawa State, Nigeria
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Abstract
Assessments of the Genetic diversity among ten local accessions of Solanum lycopersicum (Linn) from the southern senatorial district of Adamawa State, Nigeria was carried out. The study was aimed at investigating genetic diversity and elucidating the phylogenetic relationship of the local tomato accessions. Ten SSR markers were employed to investigate the genetic diversity among the tomato accessions. Eight out of ten primers i.e. (90%) were polymorphic representing loci. Total of (31) alleles were recorded and the mean number of alleles per locus was 0.2580. The lowest number of bands across genotype was two and the highest number of bands across genotypes was seven. The PIC value of SSR primers obtained ranged from 0.35 to 0.77 with the average number of polymorphic bands per primer being 23.72. While clustering among local accessions of Adamawa tomato using the unweight pair group method with arithmetic mean analysis (UPGMA), three clusters (A, B and C) were observed. The clustering however was regardless of the geographical locations where seeds were collected. It is therefore suggested that tomato accession (Tt001) with large number of fruits per truss and tomato accessions (Tt009) and (Tt010) which on the other hand had divergent phenotypic characters and had clustered closely based on molecular analysis could hybridize into a robust cultivar.
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References
Balada, C., Castro, M., Fassio, C., Zamora, A., Marchant, M., Acevedo, W. and Guzman, L. (2021) Genetic diversity and biological activity of Curcuma longa ecotypes from Rapa Nui using molecular markers. Saudi Journal of Biological Sciences. 28: 707–716.
Banhos, A., Herbeck, T., Gravena, W. and Sanaiotti, T. (2008). Genomic resources for the conservation and management of the harpy eagle (Harpiaharpyja, Falconiformes, Accipitridae). Genetics and Molecular Biology. 31(1): 146–154.
Benor, S., Zhang, M., Wang, Z. and Zhang, H. (2008). Assessment of genetic variation in tomato (Solanum lycopersicumL.) inbred lines using SSR molecular markers. Journal of Genetic Genomics. 35: 373-379.
Blanca, J., Canizares, J., Cordero, L., Pascaul, L., Diez,M.J., Nuez, F. (2012). Variations revealed by SNP genotyping and morphology provides insight into the origin of the tomato. PloS One. 7:e48198.
Bredemeijer, G., Cooke, R., Ganal, M., Peeters, R., Isaac, P., Noordijk, Y., Rendell, S., Jackson, .J, Roder, M.S., Wendehake, K., Dijcks, M., Amelaine, M., Wickaert, K., Bertrand, L. and Vosman, B. (2002). Construction and testing of a microsatellite database containing more than 500 tomato varieties. Theoretical and Applied Genetics.105: 1019–1026.
Caramante, M., Rao, R., Monti, L.M., Corrado, G. (2009). Discrimination of ‘San Marzano’ accessions: a comparison of minisatellite, CAPS and SSR markers in relation to morphological traits. Science Horticulture. 120: 560–564.
Foolad, M.R. (2007). Genome mapping and molecular breeding of tomato. International Journal of Plant Genomics. 64358.. .
Garcia-Martinez, S., Andreani, L., Garcia-Gusano, M., Geuna, F. and Ruiz, J.J. (2006) Evaluation of amplified fragment length polymorphism and simple sequence repeats for tomato germplasm fingerprinting: Utility for grouping closely related traditional cultivars. Genome. 49: 648–656.
Gonzalez, C. E., Torres, V. C. (2014). La sustentabilidadagrícola de las chinampas enelvalle de México: casoXochimilco. Revista Mexicana de Agronegocios. 34: 699-709.
Hamrick, J.L. and Loveless, M., (2019). The Genetic Structure of Tropical Tree Populations: Associations with Reproductive Biology, The Evolutionary Ecology of Plants. pp. 129–146.
He, C, Poysa, V. and Yu, K. (2003). Development and characterization of simple sequence repeat (SSR) markers and their use in determining relationships among Lycopersicon esculentum cultivars. Theoretical and Applied Genetics. 106: 363–373.
Hu, J., Wang, L. and Li, J. (2012). Comparison of genomic SSR and EST-SSR markers for estimating genetic diversity in cucumber. Biology of Plantarum. 55: 577-580.
Hussein, E.H.A., Mohamed, A.A., Attia, S., Adawy, S.S. (2016). Molecular characterization and genetic relationships among cotton genotypes using RAPD, ISSR and SSR analysis. Arab Journal of Biotechnology. 9: 313–328.
Kadams, A.M. (2010). Genetic variability: A vital tool for crop improvement. Federal University of Technology Yola, 9th Inaugural lecture. August, 2010.
Korir, N.K., Diao, W. Tao, R., Li, X, Kayesh, E., Li, A., Zhen, W. and Wang, S. (2014). Genetic diversity and relationships among different tomato varieties revealed by EST-SSRmarkers. Genetic Molecular Resource. 13 (1): 43-53.
Liu, K.J. and Muse, S.V. (2005). PowerMarker: Integrated analysis environment for genetic marker data. Bio- informatics. 21: 2121-2129.
Najaphy, A., Parchin, R.A., Farshadfar, E. (2011). Evaluation of genetic diversity in wheat cultivars and breeding lines using inter simple sequence repeat markers. Biotechnology and Biotechnology Equipments. 25: 2634–2638.
Peralta, I.E., Spooner, D.M., Knapp, S., (2008). Taxonomy of wild tomatoes and their relatives (Solanum sect. Lycopersicoides, sect. Juglandifolia, sect. Lycopersic on; Solanaceae). American Society of Plant Taxonomists. 151–160.
Rodriguez, F., Wu, F., Ane, C., Tanksley, S. and Spooner, D. M. (2009). Do potatoes and tomatoes have a single evolutionary history, and what proportion of the genome supports this history? BMC Evolutionary Biology. 9: 191-200.
Roy, S., Bhandari, V., Barman, M., Kumar, P., Bhanot, V., Arora, J.S., Singh, S. and Sharma, P. (2021). Population Genetic Analysis of the Theileriaannulata Parasites Identified Limited Diversity and Multiplicity of Infection in the Vaccine from India. Frontier of Microbiology.11:579929.
Saavadra, T.M., Figuerua, G.A. and Cauih, J.G.D. (2017). Origin and evolution of tomato production Lycopersicum esculentum in Mexico. Ciencia Rural, Santa Maria, vol. 47:03, e20160526.
Salim, M.M.R., Rashid, M.H., Hossain, M.M. and Zakaria, M. (2018). Mor¬phological characterization of tomato (Solanum lycopersicum L.) genotypes. Journal of Saudi Society of Agricultural Sciences. 19(3): 233–240.
Salunke, D.S., Jadhav, A.S., Pawar, B.D., Kale, A.A. and Chimote, V.P. (2012). Diversity Analysis of Tomato Genotypes using RAPD Markers and High-Performance Liquid Chromatography in Relation to β - Carotene Content. VEGETOS. 25(2): 95-101.
Saravanan, K.R., Rajaram, R. and Renganathan, P. (2014). Studies on genetic diversity using SSR marker associated traits in tomato genotypes(Lycopersicum esculentum L.) European Journal of Biotechnology and Bioscience. 1(5): 26-29.
Silva, G.S. and Souza, M.M. (2013). Genomic in situ hybridization in plants. Genetic Molecular Resources. 12: 2953–2965.
Srisamoot, N. and Padsri, I. (2018). Assessing genetic diversity of some Anthurium andraeanum Hort. Cut-flower cultivars using ISSR Markers. Genomics and Genetics. 11: 1-2. DOB. 10. 14456/gag
Ugonna, C.U., Jolaoso, M.A. and Onwualu, A.P. (2015). Tomato Value Chain in Nigeria: Issues,Challenges and Strategies. Journal of Scientific Research and Reports. 7(7): 501-515.
Uzun, A., Yaman, M., Pinar, H., Gok, B.D. and Gazel, I. (2021). Leaf and fruit characteristics and genetic diversity of wild fruit cerasus prostrata genotypes collected from the Central Anatolia, Turkey. Acta Science Pol Hortorum Cultus. 20: 53–62.
Wang, Z., Weber, J.L., Zhong, G., and Tanksley, S.D. (2003). Survey of plant short tandem DNA repeats. Theoretical and Applied Genetics. 88: 1−6.
Zhang, C. X., Fu J. H., Cheng, S. Z. and Lin, F. Y. (2009). Greater vegetable and fruit intake is associated with a lower risk of breast cancer among Chinese women. International Journal of Cancer. 125 (1): 181-188.
Zhou, R., Wu, Z., Cao, X., and Jiang, F.L. (2015). Genetic diversity of cultivated and wild tomatoes revealed by morphological traits and SSR markers. Genetic and Molecular Research. 14(4): 13868-138679.